Caspase-Glo® 3/7 3D Assay
Apoptosis Assay For 3D Cultures
- Quantitative measurement of caspase 3/7 activity in 3D models
- Simple add-mix-measure protocol
- Scalable to 96- and 384-well plate formats
Catalog Number:
Size
Catalog Number: G8981
Catalog Number: G8982
Catalog Number: G8983
Highly Sensitive Caspase Assay for 3D Cultures
The Caspase-Glo® 3/7 3D Assay is a homogeneous, luminescent assay that measures caspase-3 and -7 activities present in apoptotic 3D cultures. The assay reagent contains a luminogenic caspase-3/7 substrate in cell lysis buffer. A one-step addition of the reagent results in cell lysis followed by caspase cleavage of the substrate and generation of a luminescent signal. Luminescence is proportional to the amount of caspase activity present.
The assay is validated for use in a wide range of 3D cell models and is suitable for high-throughput applications. It can also be multiplexed with other homogeneous assays so you can get more data from a single well.
Note: The Caspase-Glo® 3/7 3D Assay uses the same chemistry as the Caspase-Glo® 3/7 Assay with an improved protocol designed for use with 3D models.
Learn how the Caspase-Glo® 3/7 3D Assay can be used to assess drug toxicity in organoids.
Simple Add-Mix-Measure Protocol—No Sample Prep!
The Caspase-Glo® 3/7 3D Assay uses a simple add-mix-measure protocol without the need for washes or medium removal.
Compatible with Multiple 3D Models
The Caspase-Glo® 3/7 3D Assay is validated in various 3D models including spheroids with both tight and loose junctions generated by ultra-low attachment surface chemistries and Matrigel®-embedded micromasses.
HepG2
HCT-116
HCT-116 in Matrigel®
Ideal for High-Throughput Screening
The Caspase-Glo® 3/7 3D Assay is designed for multiwell-plate formats up to 384-well, making it perfect for high-throughput screening of modulators of apoptosis in 3D cells (Z´ = 0.67). Unlike high-content imaging, the data is simple, quantitative and easy to interpret.
Multiplex with Cytotoxicity Assays in 3D Models
Tools to Monitor Biology in 3D Culture
When working with 3D culture models, choosing the right assay system is crucial. Learn about tools to monitor biology in 3D culture.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Caspase-Glo® 3/7 3D Buffer |
G898A | 1 × 10ml |
Caspase-Glo® 3/7 3D Substrate |
G899A | 1 × 1 bottle |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
Caspase-Glo® 3/7 3D Buffer |
G898B | 1 × 100ml |
Caspase-Glo® 3/7 3D Substrate |
G899B | 1 × 1 bottle |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
Caspase-Glo® 3/7 3D Buffer |
G898A | 10 × 10ml |
Caspase-Glo® 3/7 3D Substrate |
G899A | 10 × 1 bottle |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Articles
- Reproducible Drug Screening Assays Using Single Organoids
- The Promises and Challenges of 3D Models
- Verifying Cell-Based Assays for Use with 3D Models
- A CACTA-like transposon in the Anthocyanidin synthase 1 (Ans-1) gene is responsible for apricot fruit colour in the raspberry (Rubus idaeus) cultivar "Varnes"
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