Reverse Transcription System
Convenient System for Reverse Transcription Using AMV Reverse Transcriptase
Catalog Number:
Size
Catalog Number: A3500
The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in 15 minutes. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and Random Primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 separate PCR amplifications. A polyadenylated 1.2kb RNA transcript is provided as a control template for cDNA synthesis.
- Speed: Efficiently reverse transcribe poly(A)+ mRNA or total RNA in 15 minutes.
- Convenience: PCR-compatible components are provided in optimized volumes for 100 reactions.
- Positive Controls: A polyadenylated RNA transcript is provided to help troubleshoot RT-PCR parameters.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 1 × 1.2ml | 25mM | View Product |
|
Reverse Transcription 10X Buffer |
A356A | 1 × 1.4ml | View Product | |
|
Oligo(dT)15 Primer |
C110B | 1 × 50μg | 500μg/ml | View Product |
|
dNTP Mix |
C114B | 1 × 320μl | 10mM | View Product |
|
Random Primers |
C118B | 1 × 50μg | View Product | |
|
1.2kb Kanamycin Positive Control RNA |
C138A | 1 × 5μg | 0.5mg/ml | View Product |
|
AMV Reverse Transcriptase (HC) |
M900B | 1 × 1,500u | View Product | |
|
Recombinant RNasin® Ribonuclease Inhibitor |
N251A | 1 × 2,500u | 40u/μl | View Product |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
Resources
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Frequently Used With
GoTaq® Long PCR Master Mix
Hot-start master mix ideal for long-range PCR.
M4021
GoTaq® Hot Start Polymerase
Native Taq DNA polymerase with room-temperature setup for hot-start PCR.
M5001, M5005, M5006, M5008
dNTP Mix
Premixed solution of dATP, dCTP, dGTP and dTTP in water.
U1511, U1515
Random Primers
Random hexamers for universal cDNA synthesis.
C1181
