Proteasome-Glo™ Assays
Measure Chymotrypsin-, Trypsin- and Caspase-Like Activities
- Homogeneous, luminescent enzyme-based assays (not suitable for cell-based applications)
- Simple "add-mix-measure" protocol
- Results in 10–30 minutes after reagent addition
Catalog Number:
Choose an assay
Size
Catalog Number: G8621
Catalog Number: G8622
Catalog Number: G8631
Catalog Number: G8632
Catalog Number: G8641
Catalog Number: G8642
Catalog Number: G8531
Catalog Number: G8532
Fast and Simple Measurement of Protease Activity
Homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format. Adding the Proteasome-Glo™ Reagent in an "add-mix-measure" format results in proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.
The three luminogenic substrates used to monitor specific protease activities include: Suc-LLVY-aminoluciferin for chymotrypsin-like, Z-LRR-aminoluciferin for trypsin-like, and Z-nLPnLD-aminoluciferin for caspase-like activity. Each luminogenic substrate is added to a buffer system optimized for its specific proteasome activity and luciferase activity. The 26S proteasome is a 2.5MDa multiprotein complex found in all eukaryotic cells. The reagents are added to test samples containing proteasome enzyme that cleaves the substrates, releasing luciferin, which is consumed by luciferase, producing "glow-type" luminescence correlating to enzyme activity or inhibition.
The Proteasome-Glo™ 3-Substrate System consists of three homogeneous bioluminescent assays in an enzyme-based format (each of these three assays also is available separately). The simplified method minimizes handling steps and makes the assays amenable to automation. The luminescent assay format avoids inherent fluorescent background signals, providing excellent signal-to-background readings. The assays are miniaturizable to 384-well format.
Number of assays per plate
| Product | Cat.# | # Assays | Volume/Assay | Plate Size |
|---|---|---|---|---|
| Proteasome-Glo™ Chymotrypsin-Like Assay |
G8621 | 100 | 100µl | 96-well |
| 400 | 25µl | 384-well | ||
| G8622 | 500 | 100µl | 96-well | |
| 2,000 | 25µl | 384-well | ||
| Proteasome-Glo™ Trypsin-Like Assay | G8631 | 100 | 100µl | 96-well |
| 400 | 25µl | 384-well | ||
| G8632 | 500 | 100µl | 96-well | |
| 2,000 | 25µl | 384-well | ||
| Proteasome-Glo™ Caspase-Like Assay |
G8641 | 100 | 100µl | 96-well |
| 400 | 25µl | 384-well | ||
| G8642 | 500 | 100µl | 96-well | |
| 2,000 | 25µl | 384-well | ||
| Proteasome-Glo™ 3-Substrate System |
G8531 | 100* | 100µl | 96-well |
| 400* | 25µl | 384-well | ||
| G8532 | 500* | 100µl | 96-well | |
| 2,000* | 25µl | 384-well |
*For each of the 3 systems.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Proteasome-Glo™ Buffer |
G854A | 1 × 10ml |
|
Suc-LLVY-Glo™ Substrate |
G856C | 1 × 50μl |
|
Luciferin Detection Reagent |
V859A | 1 × 1 each |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Proteasome-Glo™ Buffer |
G854B | 1 × 50ml |
|
Suc-LLVY-Glo™ Substrate |
G856D | 1 × 250μl |
|
Luciferin Detection Reagent |
V859B | 1 × 1 each |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Proteasome-Glo™ Buffer |
G854A | 1 × 10ml |
|
Z-LRR-Glo™ Substrate |
G855A | 1 × 100μl |
|
Luciferin Detection Reagent |
V859A | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Proteasome-Glo™ Buffer |
G854B | 1 × 50ml |
|
Z-LRR-Glo™ Substrate |
G855B | 1 × 500μl |
|
Luciferin Detection Reagent |
V859B | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Proteasome-Glo™ Buffer |
G854A | 1 × 10ml |
|
Z-nLPnLD-Glo™ Substrate |
G857A | 1 × 50μl |
|
Luciferin Detection Reagent |
V859A | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Proteasome-Glo™ Buffer |
G854B | 1 × 50ml |
|
Z-nLPnLD-Glo™ Substrate |
G857B | 1 × 250μl |
|
Luciferin Detection Reagent |
V859B | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Proteasome-Glo™ Chymotrypsin-Like Assay |
G8621 | 1 × 10ml |
|
Proteasome-Glo™ Trypsin-Like Assay |
G8631 | 1 × 10ml |
|
Proteasome-Glo™ Caspase-Like Assay |
G8641 | 1 × 10ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Proteasome-Glo™ Chymotrypsin-Like Assay |
G8622 | 1 × 50ml |
|
Proteasome-Glo™ Trypsin-Like Assay |
G8632 | 1 × 50ml |
|
Proteasome-Glo™ Caspase-Like Assay |
G8642 | 1 × 50ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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Frequently Used With
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Ultrasensitive single-tube luminometer.
E5311
