Transfection Carrier DNA
Plasmid DNA Used to Dilute Expression or Reporter Vectors for Transfection
- Maintains the total amount of DNA used in transfection while reducing the amount of expression or reporter vector
- Lowered expression of the protein of interest can overcome potential overexpression artifacts
- Ratio of carrier DNA to reporter DNA may need to be optimized, depending on the application
- Supplied at a concentration of 1µg/μl
Catalog Number:
Size
Catalog Number: E4881
Catalog Number: E4882
Transfection Carrier DNA is a plasmid DNA used to reduce the amount of an expression vector or reporter vector in mammalian cell transfection without reducing the overall amount of DNA. Diluting reporter DNA into Transfection Carrier DNA results in lowered expression of the protein of interest, overcoming artifacts due to overexpression. This is especially relevant when strong, constitutive promoters (e.g., CMV) are used. Depending on the application, the ratio of reporter to Transfection Carrier DNA may require optimization.
This product is made from pGEM®-3Zf(–) Vector but has been purified using a method that results in low endotoxin carryover, making it more suitable for use in transfecting mammalian cells.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
Transfection Carrier DNA |
E488A | 5 × 20μg | 1μg/μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
Transfection Carrier DNA |
E488B | 2 × 100μg | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
No related resources found
Related Products
Similar Products
pNLF1 NanoLuc® Protein Fusion Vectors
Three MCS-based protein fusion vectors that express N- or C-terminal fusions with NanoLuc® luciferase or an N-terminal secreted NanoLuc® reporter.
N1351, N1361, N1371
pFN31 and pFC32 NanoLuc® Protein Fusion Flexi® Vectors
Four protein fusion Flexi® cloning vectors for generating N- or C-terminal fusions of NanoLuc® luciferase with your protein of interest.
N1311, N1321, N1331, N1341
NanoLuc® Stability Sensors for Cell Signaling
Vector systems that fuse two key signaling proteins, HIF1A and NRF2, to NanoLuc® luciferase to study the fusion protein stability.
N1381, N1391
Promoter-Driven Control NanoLuc® Luciferase Vectors
Three NanoLuc® reporter vectors with CMV, PGK or TK promoter. Co-transfect with firefly luciferase vectors for normalization in dual-reporter assays.
N1441, N1501, N1091
Frequently Used With
ViaFect™ Transfection Reagent
High-efficiency, low-toxicity transfection of many cell types, including iPS cells.
E4981, E4982
FuGENE® HD Transfection Reagent
Enhanced performance in a range of cell types, including difficult-to-transfect cell lines.
E2311, E2312
FuGENE® 6 Transfection Reagent
Proven, dependable performance. Best for everyday use with common cell lines.
E2691, E2692, E2693
ProFection® Mammalian Transfection System
Calcium phosphate-based method for stable or transient transfection of eukaryotic cells.
E1200
PureYield™ Plasmid Midiprep System
Up to 200µg of transfection-ready plasmid from 50ml cultures in 30 minutes.
A2492, A2495, A2496
pAdVAntage™ Vector
When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation.
E1711
Wizard MagneSil Tfx™ System
Transfection-quality plasmid DNA in 96-well format.
A2380
