CellTiter-Glo® Luminescent Cell Viability Assay
A Luminescent Assay to Determine the Number of Viable Cells in Culture
- A homogeneous "add-mix-measure" format reduces the number of steps
- Higher level of detection requires fewer cells per assay
- Process plates consecutively with a very stable luminescent signal
- Integrates with the MyGlo® Reagent Reader
Catalog Number:
Choose a product
Size
Catalog Number: G7570
Catalog Number: G7571
Catalog Number: G7572
Catalog Number: G7573
Catalog Number: MG1010
Cell health의 핵심 마커인 ATP를 측정하는 CellTiter-Glo®
CellTiter-Glo® Luminescent Cell Viability Assay는 대사가 활발한 세포의 지표인 ATP를 정량하여 배양된 세포에서 살아있는 세포 수를 측정하는 homogenous assay입니다. CellTiter-Glo® Assay는 multiwell 포맷에서 사용하기에 적합하도록 디자인되어 high-throughput screening (HTS), 세포 증식 및 세포 독성 분석에 이상적입니다. 이 homogenous assay 과정은 단일 시약(CellTiter-Glo® Reagent)을 serum이 포함된 배지에 직접 처리하는 방식으로 세포 washing, 배지 제거, 반복적인 피펫팅 과정이 필요하지 않습니다. 이 제품은 384-well 포맷에서 시약을 추가하고 섞은 후 10분 이내에 well당 최소 15개의 세포를 검출할 수 있습니다.
Homogenous “add-mix-measure” 형식으로 cell lysis가 진행되고, 세포 내 ATP 양에 비례하는 발광 signal이 생성됩니다. ATP 양은 배양된 세포 수와 직접적으로 비례합니다. CellTiter-Glo® Assay는 ‘glow-type’의 발광 signal을 생성하며, 이 발광 signal의 반감기는 세포 종류와 배지에 따라 다를 수 있지만 일반적으로 5시간 이상 유지됩니다. 이러한 긴 반감기는 시약 injector 없이 여러 plate를 연속적으로 또는 배치별로 처리할 수 있는 유연성을 제공합니다. 이 고유한 homogenous 방식은 여러 단계가 필요한 다른 ATP 측정 방법에서 발생할 수 있는 오류를 방지해 줍니다.
CellTiter-Glo® Luminescent Cell Viability Assay는 다양한 응용 분야에서 널리 사용되며 신뢰 받는 방법입니다.
CellTiter-Glo® Assay는 10개의 세포까지 측정할 수 있는 감도와 최대 5 log의 linear range를 제공합니다. 해당 발광 signal은 MyGlo™ Reagent Reader로 측정하였습니다.
"우리는 새로운 암 치료 타겟을 식별하기 위해 다양한 세포주에서 noncoding RNA에 대한 knockdown 실험을 수행했습니다. CellTiter-Glo® Assay는 cell viability를 평가할 때 일관되고 재현성 높은 결과를 얻는 데 매우 사용하기 쉬운 시약입니다."
Anan Quan, Research Assistant, Beth Israel Deaconess Medical Center.
"CellTiter-Glo® Assay는 세포주에서 cytopathic 효과를 유발하는 SARS-CoV 및 SARS-CoV-2와 같은 바이러스에 대한 항바이러스 활성을 가진 small molecule의 high-throughput screening에 적용하기 쉬운 매우 안정적이고 신뢰할 수 있는 시약입니다."
Dr. Colleen Jonsson, Director of the Regional Biocontainment Laboratory and Director of the Institute for the Study of Host-Pathogen Systems, University of Tennessee Health Science Center.
Jonsson 박사의 항바이러스제 연구에 대한 자세한 내용은 여기에서 확인하세요.
Notes
96-well 포맷에서 assay당 CellTiter-Glo® Reagent 100µl를 사용하는 경우, Cat.# G7570은 100 assay; Cat.# G7571, G7572는 1,000 assay; Cat.# G7573은 10,000 assay를 수행할 수 있습니다.
384-well 포맷에서 assay당 CellTiter-Glo® Reagent 25µl를 사용하는 경우, Cat.# G7570은 400 assay; Cat.# G7571, G7572는 4,000 assay; Cat.# G7573은 40,000 assay를 수행할 수 있습니다.
Customer Testimonials: Advantages of CellTiter-Glo
Bioluminescence 리소스 센터
발광의 원리와 형광과 어떤 차이점을 알아보고 발광을 어떻게 연구에 활용할 수 있는지에 대해 자세히 알아보세요.
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
CellTiter-Glo® Substrate (lyophilized) |
G755A | 1 × 1 vial |
|
CellTiter-Glo® Buffer |
G756A | 1 × 10ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
CellTiter-Glo® Substrate (lyophilized) |
G755A | 10 × 1 vial |
|
CellTiter-Glo® Buffer |
G756A | 10 × 10ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
CellTiter-Glo® Substrate (lyophilized) |
G755B | 1 × 1 vial |
|
CellTiter-Glo® Buffer |
G756B | 1 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
CellTiter-Glo® Substrate (lyophilized) |
G755B | 10 × 1 vial |
|
CellTiter-Glo® Buffer |
G756B | 10 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
MyGlo™ Reagent Reader
|
MG1000 | 1 × 1 each | |
CellTiter-Glo® 2.0 Reagent |
G9241 | 1 × 10ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.
Resources
Articles
- Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins
- Impact of aerosols on liver xenobiotic metabolism: A comparison of two methods of exposure
- Tolerance to colibactin correlates with homologous recombination proficiency and resistance to irinotecan in colorectal cancer cells
- Comparative characterization of different molecular formats of bispecific antibodies targeting EGFR and PD-L1
- Overcoming preclinical safety obstacles to discover GDC-2394: A potent and selective NLRP3 inhibitor
- Elevated expression of miR-494-3p is associated with resistance to osimertinib in EGFR T790M-positive non-small cell lung cancer
- Insights into the modular design of kinase inhibitors and application to Abl and Axl
- Metformin attenuates rotenone-induced oxidative stress and mitochondrial damage via the AKT/Nrf2 pathway
- Endogenous retroviruses mediate transcriptional rewiring in response to oncogenic signaling in colorectal cancer
- A CACTA-like transposon in the Anthocyanidin synthase 1 (Ans-1) gene is responsible for apricot fruit colour in the raspberry (Rubus idaeus) cultivar "Varnes"
- Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals
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