Intracellular TE Nano-Glo® Vivazine® Substrate/Extracellular NanoLuc® Inhibitor
For use with NanoBRET® Target Engagement Intracellular Assays over Multiple Hours
- Supplies the Nano-Glo® Vivazine® Substrate and Extracellular NanoLuc® Inhibitor
- Optimal for experiments >2 hours
- Ensures measurements originate from inside intact cells
Catalog Number:
Size
Catalog Number: N2200
Catalog Number: N2201
Intracellular TE Nano-Glo® Vivazine® Substrate/Extracellular NanoLuc® Inhibitor
The Intracellular TE Nano-Glo® Vivazine® Substrate/Extracellular NanoLuc® Inhibitor is designed for use with NanoBRET® Target Engagement (TE) Assays, which measure compound binding at select target proteins in intact cells in real time. The NanoBRET® TE Assay uses four key components: an expressed cellular target protein that is fused to the bright NanoLuc® luciferase; a cell-permeable fluorescent tracer that specifically binds to the target protein; a substrate for NanoLuc® luciferase; and a cell-impermeable inhibitor for NanoLuc® luciferase.
The Intracellular TE Nano-Glo® Vivazine® Substrate/Extracellular NanoLuc® Inhibitor is designed for NanoBRET® TE measurements in cells lasting longer than 2 hours. The Nano-Glo® Vivazine® Substrate is an extended-lifetime NanoLuc® substrate designed to provide a bright luminescent signal for end-point measurements or real-time kinetic experiments. The Extracellular NanoLuc® Inhibitor is a cell-impermeable molecule designed to quench NanoLuc® signal derived from the extracellular environment or compromised cells.
Looking for more information on NanoBRET® Kinase Target Engagement?
Explore the TechnologySpecifications
Catalog Number:
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
Resources
No related resources found
Related Products
Frequently Used With
NanoBRET® Target Engagement Kinase Assays
Directly measure kinase-ligand binding affinity, occupancy and residence time in live cells.
N2520, N2521, N2540, N2501, N2500, N2530, N2600, N2601, N2620, N2621, N2630, N2631, N2640, N2641, N2650, N2651, NF1001, N2810, N2820, N2830, N2840, N2850, NF1200
NanoBRET® Target Engagement BET BRD Assays
Directly measure BET bromodomain-test compound affinity and residence time in live cells.
N2131, N2180, N2130, N2140
NanoBRET® Target Engagement HDAC Assays
Directly measure HDAC-test compound affinity and residence time in live cells.
N2080, N2081, N2170, N2090
