Lumit® Immunoassay Cellular Systems

No-Wash, One-Plate Immunoassay for Kinase Pathway Analysis

  • Set 1: Lumit® Anti-Mouse Ab-LgBiT/Lumit® Anti-Rabbit Ab-SmBiT
  • Set 2: Lumit® Anti-Mouse Ab-SmBiT/Lumit® Anti-Rabbit Ab-LgBiT
  • Starter Kit: Includes all four Set 1 and Set 2 Lumit® Secondary Antibodies; primary antibodies supplied by the user
  • Application Notes available for validated targets
  • New Lysis Buffer II is available with enhanced performance across a wide range of protein targets. Request a sample.

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Catalog number selected: W1201

$ 394.00
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Lumit® Immunoassay Cellular Systems
Set 1/100 assays
$ 394.00
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Fast Quantification of Phosphorylated or Total Protein

The Lumit® Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell lysates. The assay can be adapted to detect any phosphoprotein, total protein or small molecule of interest, provided that appropriate primary antibodies (supplied by the user) are available.

Also available are Complete Assays, which include sets of phospho-specific antibodies for several important signaling nodes—pERK, pAKT, pSTAT3, pIκBα and pBTK. These are available as early access materials.

To save you time, we have evaluated a list of common targets using commercially available primary antibodies. Our collection of application notes contains protocols, example data, optimized conditions and ordering information for each primary antibody.

List of Validated Targets
Cellular Target Signaling Pathway Total Protein Protocol Phosphorylated Protein Protocol Lumit Immunoassay Cellular System Lumit Cat.# Application Note

4E-BP1 (Human)

mTor No Yes (Ser 65) Set 2 W1331 Download

AKT (Human)

PI3K/mTOR/AKT Yes Yes (Ser 473) Set 1 W1201 Download

BCL6 (Human)

B-cell signaling Yes No Set 1 W1201 Download

β-catenin (Human)

WNT Yes Yes (Thr 41/ Ser 45) Set 2 W1331 Download

BTK (Human)

B-cell Receptor (BCR) No Yes (Tyr 223) Set 1 W1201 Download

c-Jun (Human)

JNK No Yes (Ser 63) Set 1 W1201 Download

CREB (Human)

PKA/CREB Yes Yes (Ser 133) Set 1 W1201 Download

ER (Estrogen Receptor - Human)

Estrogen Yes No Set 1 W1201 Download

ERK1 (Human)

ERK No Yes (Thr 202) Set 1 W1201 Download

IκBα (Human)

NF-κB
Yes Yes (Ser 32) Set 1 W1201 Download

JNK (Human)

JNK No Yes (Thr 183/Tyr 185) Set 1 W1201 Download

p65 (Human)

NF-κB
Yes Yes (Ser 536) Set 2 W1331 Download

Rb (Retinoblastoma Tumor Suppressor - Human)

Cell Cycle Yes Yes (Ser 780) Set 2 W1331 Download

Rb (Retinoblastoma Tumor Suppressor - Human)

Cell Cycle Yes Yes (Ser 807/811) Set 2 W1331 Download

Smad1 (Human)

BMP Yes Yes (Ser 463/465) Set 1 W1201 Download

Smad2 (Human)

TGF-β Yes Yes (Ser 465/467) Set 2 W1331 Download

STAT1 (Human)

JAK/STAT Yes Yes (Tyr 701) Set 1 W1201 Download

STAT1 (Human)

JAK/STAT Yes Yes (Ser 727) Set 1 W1201 Download

STAT2 (Human)

JAK/STAT No Yes (Tyr 690) Set 1 W1201 Download

STAT 3 (Human)

JAK/STAT Yes Yes (Tyr 705) Set 1 W1201 Download

How the Lumit® Immunoassay Cellular System Works

Lumit® Immunoassay Cellular Systems include Lumit® Secondary Antibodies (rabbit and mouse; Lumit® Anti-Goat Antibody conjugates available separately), which are conjugated with NanoBiT® subunits: LgBiT and SmBiT. Binding of the antibody complexes brings LgBiT and SmBiT into close proximity to form an active NanoLuc® luciferase that produces light in proportion to the amount of target analyte.
See All Lumit® Secondary Antibodies
lumit immunoassay cellular system phosphoprotein detection

Simple One-Plate Protocol, No Wash Steps!

The Lumit® Immunoassay Cellular System does not require media removal or lysate transfer. The protocol simply involves addition of the labeled antibodies to the sample, addition of the detection reagent and reading of the luminescent signal—all in a single plate!

how-the-lumit-immunoassay-works-protocol

Lumit® Immunoassay vs. Western Blot

Lumit® Immunoassays are easier and much faster than traditional Western blots while generating comparable data.

Lumit immunoassay Wester blot protocol comparison
phospho-IkB lumit immunoassay vs Western blot
Total IkB lumit immunoassay vs Western blot

Quantitative Detection of Total and Phosphorylated Proteins

lumit-phospho-assay-ikb-p65

Using Lumit® Immunoassay Cellular Systems for Screening Novel Compounds in a Drug Discovery Process

Here, Dr. Tracey Martin from Cardiff University School of Medicine describes use of Lumit® Immunoassays in her work studying the motility protein N-WASP. The project involves screening 150 novel compounds to assess their effects on N-WASP expression and phosphorylation status. Multiple concentrations of each compound are tested in different cell types, generating a large number of samples. Originally the team were using Western blotting to look at N-WASP levels, however this was found to be too expensive and both labor and time-intensive.

Download Case Study

 

See More Data: This peer-reviewed paper published in Nature Communications Biology demonstrates how Lumit® Immunoassays were used to monitor multiple signaling pathway activation through specific nodes of phosphorylation (pIκBα, pAKT and pSTAT3).

Read the Paper
lumit-immunoassay-nature-communications-biology-paper

Specifications

You are viewing: W1201 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944C 1 × 50μl

Lumit® Detection Substrate C

VB103A 1 × 200μl

Lumit® Immunoassay Buffer C, 10X

VB115A 1 × 1.5ml

Lumit® Anti-Mouse Ab-LgBiT

W102A 1 × 30μl

Lumit® Anti-Rabbit Ab-SmBiT

W103A 1 × 30μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A-C 1 × 10μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1202 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 1 × 500μl

Lumit® Detection Substrate C

VB103B 2 × 1ml

Lumit® Immunoassay Buffer C, 10X

VB115B 1 × 15ml

Lumit® Anti-Mouse Ab-LgBiT

W102B 1 × 300μl

Lumit® Anti-Rabbit Ab-SmBiT

W103B 1 × 300μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A-C 2 × 10μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1203 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 10 × 500μl

Lumit® Detection Substrate C

VB103B 20 × 1ml

Lumit® Immunoassay Buffer C, 10X

VB115B 10 × 15ml

Lumit® Anti-Mouse Ab-LgBiT

W102B 10 × 300μl

Lumit® Anti-Rabbit Ab-SmBiT

W103B 10 × 300μl

Antibody Dilution Buffer

W108A 10 × 1ml

GF-AFC Substrate

G608A-C 20 × 10μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1331 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944C 1 × 50μl

Lumit® Detection Substrate C

VB103A 1 × 200μl

Lumit® Immunoassay Buffer C, 10X

VB115A 1 × 1.5ml

Lumit® Anti-Rabbit Ab-LgBiT

W104A 1 × 30μl

Lumit® Anti-Mouse Ab-SmBiT

W105A 1 × 30μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A-C 1 × 10μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1332 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 1 × 500μl

Lumit® Detection Substrate C

VB103B 2 × 1ml

Lumit® Immunoassay Buffer C, 10X

VB115B 1 × 15ml

Lumit® Anti-Rabbit Ab-LgBiT

W104B 1 × 300μl

Lumit® Anti-Mouse Ab-SmBiT

W105B 1 × 300μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A-C 2 × 10μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1333 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 10 × 500μl

Lumit® Detection Substrate C

VB103B 20 × 1ml

Lumit® Immunoassay Buffer C, 10X

VB115B 10 × 15ml

Lumit® Anti-Rabbit Ab-LgBiT

W104B 10 × 300μl

Lumit® Anti-Mouse Ab-SmBiT

W105B 10 × 300μl

Antibody Dilution Buffer

W108A 10 × 1ml

GF-AFC Substrate

G608A-C 20 × 10μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1220 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944C 2 × 50μl

Lumit® Detection Substrate C

VB103A 2 × 200μl

Lumit® Immunoassay Buffer C, 10X

VB115A 2 × 1.5ml

Lumit® Anti-Mouse Ab-LgBiT

W102A 1 × 30μl

Lumit® Anti-Rabbit Ab-SmBiT

W103A 1 × 30μl

Lumit® Anti-Rabbit Ab-LgBiT

W104A 1 × 30μl

Lumit® Anti-Mouse Ab-SmBiT

W105A 1 × 30μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A-C 1 × 10μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

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