Maxwell® HT 96 gDNA Blood Isolation System
Automated DNA Purification from Whole Blood, Cells and Tissue
- High yields of pure DNA from pristine and challenged or hemolysed blood
- Robust protocol compatible with blood stored in EDTA, citrate, and heparin
- Highly pure DNA is ready for qPCR, NGS, arrays
Catalog Number:
Size
Catalog Number: A2670
Catalog Number: A2671
Ready-to-Go Blood DNA Kit, Expert Support Available
The Maxwell® HT 96 gDNA Blood Isolation System is compatible with automation on all major platforms, including Hamilton Star/Nimbus, Tecan Freedom Evo/Fluent, Biomek i5/i7, Eppendorf epMotion, and particle movers such as KingFisher.
With decades of experience in building personalized automation solutions, we can help you customize the scale, application, and chemistry, regardless of platform, exactly to your needs—the only limit is your imagination.
Automated DNA Extraction from Blood or Saliva
- Simple, automation-friendly method
- No centrifugation or vacuum required
- No organic solvents
- High yield and purity
The Maxwell® HT 96 gDNA Blood Isolation System provides a simple and reliable method for rapid isolation of genomic DNA from blood or saliva in a multiwell format. DNA may be purified from blood and Oragene®•Discover sample collection devices. The purified DNA can be used directly in PCR, microarrays and next-generation sequencing applications.
DNA yields of up to 12µg are expected from input blood volumes of 350µl, depending on the white blood cell count of the sample. Saliva samples can have variable amounts of gDNA, and up to 18µg or more of DNA may be recovered from a 700µl Oragene® collection device sample.
Example Data: Automated DNA Extraction From Blood, Saliva & Tissue
These application notes contain protocols and example data using the Maxwell® HT gDNA Purification System to purify DNA from blood, saliva and tissue using various robotic systems.
- Purification of DNA from Saliva in Oragene® Collection Devices using the Hamilton STAR Instrument
- DNA Purification from Whole Blood Stabilized in Various Collection Tubes (Tecan Freedom EVO™ or Beckman Biomek® FX)
- DNA Purification from Cattle Ear Punches and Hair Using Maxwell® HT 96 gDNA Blood Isolation System on the KingFisher™ Flex
For more information on protocols for purification from cells and tissue, please contact us.
Your Purification Solution, Simplified
Our team of automation experts will help you choose an optimized high-throughput solution that meets your laboratory’s needs. We can implement Maxwell® HT chemistries on a variety of sample types and instrumentation platforms. Contact us for a consultation about choosing the right purification chemistry or customizing a purification chemistry for additional sample types.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
| Binding Buffer (BBA) | A174B | 1 × 50ml | View Product | |
Resin |
A175B | 1 × 5.5ml | View Product | |
Wash Buffer (WBA) |
A176B | 1 × 85ml | View Product | |
10mM EDTA (pH 8.0) |
A263A | 1 × 10ml | View Product | |
|
Proteinase K (PK) Solution |
A505C | 1 × 5.5ml | View Product | |
|
RNase A Solution |
A797C | 1 × 1ml | 4mg/ml | View Product |
25mM Tris-HCl (pH 8.0) |
A2641 | 1 × 60ml | View Product | |
Cell Lysis Buffer (CLD) |
A173B | 1 × 40ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
Binding Buffer (BBA) |
A174C | 1 × 200ml | View Product | |
Resin |
A175B | 4 × 5.5ml | View Product | |
Wash Buffer (WBA) |
A176C | 1 × 350ml | View Product | |
10mM EDTA (pH 8.0) |
A263A | 1 × 10ml | View Product | |
25mM Tris-HCl (pH 8.0) |
A2641 | 1 × 60ml | View Product | |
|
Proteinase K (PK) Solution |
A505D | 1 × 23ml | View Product | |
RNase A Solution |
A797E | 1 × 5ml | 4mg/ml | View Product |
Cell Lysis Buffer (CLD) |
A173C | 1 × 160ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
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Heat Block Adapter
Designed for a 2.2ml deep-well 96-well plate (Cat. # V6781) with Maxwell® HT 96 gDNA Blood Isolation System (Cat.# A2670, A2671).
A2661
