FuGENE® HD Transfection Reagent
Better Transfection for Challenging Cell Lines
- Difficult-to-transfect cell lines
- Transfection of primary cells
- Protein and virus production
Catalog Number:
Size
Catalog Number: E2311
Catalog Number: E2312
What Is FuGENE® HD Transfection Reagent?
FuGENE® HD is a non-liposomal, multi-component transfection reagent designed to deliver nucleic acids into eukaryotic cells with high efficiency and low toxicity. It is manufactured without animal-derived components and is compatible with serum-containing and chemically defined media. This makes it suitable for applications where physiological relevance and reproducibility matter — from basic research to protein and virus production.
Transfection reagents vary widely in how much they stress the cells they deliver into. In a head-to-head comparison of four reagents, FuGENE® HD produced the fewest off-target transcriptional changes (Jacobsen et al., 2009).
Which Cell Types Can Be Transfected with FuGENE® HD?
FuGENE® HD supports transfection of a broad range of cell types, including many lines considered difficult to transfect. Validated cell types include:
- primary cells
- cancer model cell lines
- stem cells
- insect cells
The Transfection Assistant helps you optimize transfection protocols using information from published literature. Find citations on transfection of specific cell types, including the amount of nucleic acid and reagent-to-DNA ratio used.
Why Choose FuGENE® HD Over Other Transfection Reagents?
FuGENE® HD outperforms common lipid-based transfection reagents on two metrics that matter most for transfection: high efficiency and low toxicity.
Two practical advantages set it apart:
- No need to reduce serum before transfection. Cells can be transfected in up to 100% serum, so serum-dependent and primary cells can be transfected without first adapting them to low-serum conditions.
- Reagent/DNA complexes do not need to be washed away post-transfection. This reduces handling steps and stress on cells.
High Expression, Low Toxicity
| FuGENE® HD Benefits | Why it matters |
|---|---|
| Efficient DNA delivery | More cells take up your DNA, giving a strong signal for downstream assays |
| Low toxicity | Keeps cells healthy, so your results reflect your gene, not the reagent |
| Transfects in up to 100% serum | Works with serum-dependent and primary cells without switching them to low-serum conditions first |
| No wash or medium change after transfection | Fewer hands-on steps and less disruption to your cells |
| No animal-derived components; compatible with chemically defined media | Fits animal-free and chemically defined media workflows |
Unrivaled Transfection Performance
Transfection of Stem Cells
“FuGENE® HD resulted in the highest stable transfection rate, estimated by antibiotic selection, with plasmids containing genes under the control of the EF1alpha or PGK promoters”.
Liu J., et al. (2009) Stable transgene expression in human embryonic stem cells after simple chemical transfection. Mol. Reprod. Dev. (2009) 76, 580-6.
Transfection of Cancer Cell Models
FuGENE® HD Transfection Reagent lets you work in your cancer model. Use the FuGENE® HD protocol database to see if transfection conditions for your cell line are available. The database includes over 25 cancer models and provides transfection protocols specific for each cell line.
Cellular Imaging
High expression levels and the low toxicity of FuGENE® HD can bring better performance for imaging experiments. Read more about the low toxicity of FuGENE® HD in:
Antczuk et al. (2014) A High Content Assay to Assess Cellular Fitness. Comb Chem High Throughput Screen. 17(1), 12–24.
Protein Production
FuGENE® HD is made without animal products and is compatible with defined media. This means you can conduct experiments in mammalian and eukaryotic cells with confidence.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
FuGENE® HD Transfection Reagent |
E231A | 1 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
FuGENE is a registered trademark of Fugent, L.L.C., USA.
FuGENE® HD is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
FuGENE® HD Transfection Reagent |
E231A | 5 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
FuGENE is a registered trademark of Fugent, L.L.C., USA.
FuGENE® HD is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.
Resources
Articles
- Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editing
- G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker, promotes proliferation and invasion of triple negative breast cancer
- Discovery of an orally bioavailable and selective PKMYT1 inhibitor, RP-6306
- An interaction between SLC35A1 and ST3Gal4 is differentially affected by CDG-causing mutations in the SLC35A1 gene
- Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins
- Endogenous retroviruses mediate transcriptional rewiring in response to oncogenic signaling in colorectal cancer
- In vitro structure–activity relationships and forensic case series of emerging 2‑benzylbenzimidazole ‘nitazene’ opioids
Compare Products
| FuGENE® HD | FuGENE® 4K | FuGENE® 6 | |
|---|---|---|---|
| Best suited for | Difficult-to-transfect cells: cancer models, insect cells, stem cells | High-yield protein expression and viral production | Routine transfection of common cell lines |
| Cell type range | Broad range, including insect and stem cells | Challenging and routine mammalian cell lines | Broad range of common mammalian cell lines |
| Serum compatibility | Up to 100% serum | With or without serum (complexes formed serum-free) | In presence of serum |
| Optimal reagent:nucleic acid ratio | 1.5:1–4:1 | 3:1–6:1 (4:1 recommended) | 1.5:1–3:1 (up to 6:1) |
Similar Products
FuGENE® 6 Transfection Reagent
Proven, dependable performance. Best for everyday use with common cell lines.
E2691, E2692, E2693
ViaFect™ Transfection Reagent
High-efficiency, low-toxicity transfection of many cell types, including iPS cells.
E4981, E4982
ProFection® Mammalian Transfection System
Calcium phosphate-based method for stable or transient transfection of eukaryotic cells.
E1200
Frequently Used With
NanoBRET® Target Engagement Selectivity DNA Controls
Quantitatively measure intracellular compound fractional occupancy against many Kinase in a single experiment.
NP1000
TransfectNow™ HEK293 Cells
Transfection-ready cells---no cell culture required. Simply thaw, dilute and transfect.
NC1001, NC1002
PureYield™ Plasmid Midiprep System
Up to 200µg of transfection-ready plasmid from 50ml cultures in 30 minutes.
A2492, A2495, A2496
Bright-Glo™ Luciferase Assay System
A sensitive reporter assay for continuous-processing on robotic systems.
E2610, E2620, E2650
ONE-Glo™ Luciferase Assay System
Robust firefly luciferase assay with improved performance for high- and ultrahigh-throughput applications.
E6110, E6120, E6130
pmirGLO Dual-Luciferase miRNA Target Expression Vector
Evaluate microRNA (miRNA) activity by inserting target sites adjacent to the firefly luciferase gene. Normalize with Renilla luciferase.
E1330
Product Citations
Recent publications that mention the use of this product.
Frequently Asked Questions
Does FuGENE® HD require serum-free conditions for transfection?
No. FuGENE® HD works in serum-containing media and can transfect cells in up to 100% serum. There is no need to switch to serum-free media before or during the transfection step, which simplifies the protocol and maintains physiologically relevant culture conditions.
Is FuGENE® HD suitable for generating stable cell lines?
Yes. FuGENE® HD has been used to generate stable transgenic lines in multiple cell types, including human embryonic stem cells. In one study using a human ES cell line, FuGENE® HD gave the highest stable transfection rate among the chemical reagents tested when paired with EF1α or PGK promoter-driven constructs (Liu et al., 2009).
How do I find the optimal transfection conditions for my cell line?
The Transfection Assistant helps you optimize transfection protocols using information from published literature. Search by cell line name to find the amount of nucleic acid and reagent-to-DNA ratio used for specific plate formats. For more information on optimizing transfection efficiency, visit our Transfection Guide.
Does FuGENE® HD contain animal-derived components?
No. FuGENE® HD is manufactured without animal-derived products, making it compatible with chemically defined media systems and suitable for applications where animal-free reagents are required.
