Maxwell® RSC ccfDNA Plasma Kit
Automated ccfDNA Purification with a Simple Three- or Five-Step Protocol
- Purifies circulating cell-free DNA from up to 8ml or more of plasma
- Consistently high yields of amplifiable ccfDNA
- Simple, three- or five-step protocol with minimal preprocessing
Catalog Number:
Choose a Sample Input Volume
Catalog Number: AS1480
Catalog Number: AS1840
Easy-to-Use Protocols Yield High-Quality, Amplifiable ccfDNA
The Maxwell® RSC ccfDNA Plasma Kits are used with Maxwell® RSC Instruments to purify circulating cell-free DNA (ccfDNA) or circulating tumor DNA (ctDNA) from plasma samples. The Maxwell® RSC ccfDNA Plasma Kit (Cat.# AS1480) offers a fully-automated extraction workflow for 0.2–1.0ml plasma samples in approximately 70 minutes. The Maxwell® RSC ccfDNA LV Plasma Kit (Cat.# AS1840) offers greater flexibility with a semi-automated extraction workflow for 2-4ml plasma samples, or 4ml-8ml plasma samples with purchase of additional Binding Buffer (BBC) (Cat.# MC1361). The purified cell-free DNA from the Maxwell® RSC ccfDNA Plasma Kits is of high quality with little genomic DNA contamination and is ready for use in downstream applications such as qPCR, next-generation sequencing (NGS) and digital PCR.
The Maxwell® RSC ccfDNA Plasma Kits are compatible with the Maxwell® RSC Instrument, which processes from 1 to 16 samples simultaneously, and the Maxwell® RSC 48 Instrument, which processes from 1 to 48 samples simultaneously. The Maxwell® RSC ccfDNA Plasma Kits are also compatible for use on the Maxwell® CSC Instrument in RUO Mode, or Maxwell® CSC 48 Instrument in RUO Mode.
No Clogging Worries with a Paramagnetic Particle Mover
Because the Maxwell® RSC instruments are magnetic particle movers, not liquid handlers, they offer advantages over other automated systems. There is minimal risk of cross-contamination because no liquid handling or splashing happens during sample processing. With no clogs and fewer breakdowns, there are fewer disruptions to your workflow.
Process Large-Volume Plasma with Minimal User Intervention
Simple Processing Protocol
The prefilled cartridges and absence of preprocessing steps make it easy to purify high-quality ccfDNA with a simple three- or five-step protocol. In contrast, the lengthy Qiagen protocol requires multiple complicated processing steps.
Simple protocol saves time. Side-by-side comparison of the protocol steps for the Maxwell® RSC ccfDNA Plasma Kits and the QIAamp® Circulating Nucleic Acid Kit.
High Yields of Amplifiable DNA Let You Move to Downstream Assays Quickly
High-quality ccfDNA is ready-to-use in downstream assays including digital PCR and NGS.
Circulating cell-free DNA yields are equivalent to those from the QIAamp® Kit. DNA was purified from 1ml of plasma using the Maxwell® RSC ccfDNA Plasma Kit and the Qiagen QIAamp® Circulating Nucleic Acid Kit. Isolated ccfDNA was eluted in 80µl, and yield was quantified by qPCR.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
Maxwell® RSC Cartridge (RSCG) |
AS148A | 48 × 1 each | |
Elution Tubes (0.5ml) |
AS620A | 1 × 50/pk | View Product |
Elution Buffer |
MC139B | 1 × 20ml | |
RSC Plungers |
AS1670 | 1 × 48/pack | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
RSC Plungers |
AS1670 | 1 × 48/pack | View Product |
Maxwell® RSC Cartridge (RSCP) |
AS184A | 48 × 1 each | |
Elution Tubes (0.5ml) |
AS620A | 1 × 50/pk | View Product |
Binding Buffer (BBC) |
MC1361 | 1 × 200ml | View Product |
Maxwell® Resin E |
MC148A | 1 × 10ml | |
PCR Elution Buffer (EBE) |
MC1511 | 1 × 5ml | View Product |
NGS Elution Buffer (EBF) |
MC1521 | 1 × 5ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Software and Firmware
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