FcγRI ADCP Reporter Bioassay, Core Kit Technical Manual
Instructions for Use of Product(s)
GA1341, GA1345, GA1351, GA1355
Literature # TM695
The FcγRI ADCP Reporter Bioassay, Core Kit, is a bioluminescent cell-based assay that can be used to measure the potency and stability of antibodies and other biologics with Fc domains that bind and activate FcγRI. The assay consists of a genetically engineered Jurkat T cell line that expresses the high-affinity human FcγRI and a luciferase reporter. The cell line is provided in a thaw-and-use format, as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation.
When cocultured with a target cell and relevant antibody, the FcγRI Effector Cells bind the Fc domain of the antibody, resulting in FcγRI signaling and response element-mediated luciferase activity. The bioluminescent signal is detected and quantified using the Bio-Glo™ Luciferase Assay System and a standard luminometer such as the GloMax® Discover System.
This Technical Manual is relevant for Cat.# GA1341 and GA1345; GA1351 and GA1355 (Korea).
Summary of Changes
The following changes were made to the 5/25 revision of this document:
1. Revised text about the label in Section 3.
2. Updated fonts.
3. Made miscellaneous text edits.
Revised 5/25.