리포터 바이러스(Reporter Viruses)

NanoLuc® luciferase reporter gene과 HiBiT tag은 크기가 작기 때문에 packaging이나 enzyme 활성에 영향을 주지 않고 바이러스 게놈에 삽입할 수 있어 생체 내 바이러스 과정을 쉽게 모니터링할 수 있습니다.

Small reporter, 바이러스 연구를 발전시키다.

재조합 리포터 바이러스(recombinant reporter vírus)는 세포와 동물 모델에서 바이러스 life cycle과 치사율에 대한 이해를 높이는 중요한 tool입니다. 리포터 바이러스를 사용함으로 시간 변화에 따른 감염을 쉽게 추적하고 바이러스의 세포 내 진입 및 복제와 같은 현상을 정량화할 수 있습니다. 크기가 큰 리포터 유전자(예시. firefly luciferase)를 바이러스 게놈에 삽입하면 바이러스 과정에 영향을 주는 경우가 종종 발생하지만 크기가 작은 NanoLuc® luciferase와 HiBiT tag은 바이러스에 영향을 주지 않고 바이러스 게놈에 안정적으로 삽입될 수 있습니다. 아래의 article에서는 NanoLuc®과 NanoBiT® technology로 제작된 안정적인 리포터 바이러스를 이용하여 in vitro와 in vivo에서 바이러스 병원성 및 전파를 조사한 최근 연구를 소개합니다.

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Luciferase Size Comparison

NanoLuc® luciferase를 이용한 재조합 리포터 바이러스

NanoLuc® luciferase를 이용하여 많은 재조합 바이러스와 vírus-like particles (VLPs)이 만들어지고 있습니다.
재조합 NanoLuc® 바이러스 관련 논문 검색은 아래 검색창에 바이러스 이름을 입력해 주세요.

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Additional Resources

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